rabbit anti p70 s6k thr389 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
rabbit anti p70 s6k thr389
Rabbit Anti P70 S6k Thr389, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+s6k/pmc13016079-15-0-4
Average 86 stars, based on 1 article reviews
Rabbit Anti P70 S6k Thr389, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+s6k/pmc13016079-15-0-4
Average 86 stars, based on 1 article reviews
rabbit anti p70 s6k thr389 - by Bioz Stars,
2026-09
86/100 stars
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Incubation:Article Title: Central Zika virus infection causes hypothalamic inflammation and persistent insulin resistance in adult mice. Article Snippet: 30–40 μg of protein were loaded in precast NuPAGE Novex 4–12% polyacrylamide gradient gels (Invitrogen, ThermoFisher, MA), along with molecular weight standards (Precision Plus from Bio-Rad, CA), and electrophoresis was carried out at 125 V in a 3-(N-morpholino) propanesulfonic acid (MOPS) running buffer (in mmol/L: 50 MOPS, 50 Tris Base, 1 EDTA, 0.1% SDS, pH 7.7) and were electrotransferred to PVDF membrane for 1 h 15min at 200mA. .. Blots were blocked for 1 h with 5% BSA in TweenTris buffer solution at room temperature and were incubated overnight at 2 °C with rabbit anti-insulin receptor β (1:500, Cell Signaling, #3020), rabbit anti-IRS1pSer307 (1:500, Cell Signaling #2381), Blocking Assay:Article Title: Central Zika virus infection causes hypothalamic inflammation and persistent insulin resistance in adult mice. Article Snippet: 30–40 μg of protein were loaded in precast NuPAGE Novex 4–12% polyacrylamide gradient gels (Invitrogen, ThermoFisher, MA), along with molecular weight standards (Precision Plus from Bio-Rad, CA), and electrophoresis was carried out at 125 V in a 3-(N-morpholino) propanesulfonic acid (MOPS) running buffer (in mmol/L: 50 MOPS, 50 Tris Base, 1 EDTA, 0.1% SDS, pH 7.7) and were electrotransferred to PVDF membrane for 1 h 15min at 200mA. .. Blots were blocked for 1 h with 5% BSA in TweenTris buffer solution at room temperature and were incubated overnight at 2 °C with rabbit anti-insulin receptor β (1:500, Cell Signaling, #3020), rabbit anti-IRS1pSer307 (1:500, Cell Signaling #2381), Western Blot:Article Title: Lysosomal and mTORC1 signaling dysregulation underpin the pathology of spastic paraplegia type 80 Article Snippet: Finally, imaging was performed using Zeiss LSM 800 or Olympus FV3000 microscope. .. The following antibodies were used for western blotting analysis: Rabbit anti-LAMP1 (1:3000, 21997-1-AP, Proteintech ), Mouse anti-LAMP2 (1:3000, 66301-1-Ig, Proteintech), Rabbit anti-p62 (1:2000, 18420-1-AP, Proteintech), Rabbit anti-LC3B (1:1000, L7543, Merck), Rabbit anti-UBAP1 (1:5000, 12385-1-AP, Proteintech), Rabbit anti-VPS37A (1:5000, 11870-1-AP, Proteintech), Mouse anti-mTOR (1:5000, 66888-1-Ig, Proteintech), Mouse anti-p-mTOR (1:5000, 67778-1-Ig, Proteintech), Rabbit anti-TFEB (1:2000, 13372-1-AP, Proteintech), Rabbit anti-p-TFEB (1:2000, 87932T, CST), Article Title: MRG15 decline in aged/injured MuSCs hinders regeneration via differentiation defects. Article Snippet: Protein from cells or tissues was extracted by using RIPA buffer (P0013B, Beyotime) supplemented with Protease Inhibitor Cocktail (5892791001, Roche) and Phosphatase Inhibitor Cocktail (4906837001, Roche) immediately before use and the protein concentration was determined using a Pierce Coomassie Plus (Bradford) assay kit (23238, Thermo Fisher Scientific). .. The following antibodies and dilutions were used for Western blot analysis: rabbit anti-MRG15 (1:1000, 55257–1-AP, Proteintech), mouse anti-MyoG (1:1000, F5D, DSHB), mouse anti-MyoD1 (1:1000, M3512, Dako), mouse anti-MYH (sc-376157, Santa Cruz), mouse anti-TIP60 (1:1000, 10827–1-AP, Proteintech), mouse anti-P300 (1:1000, 83078–5-RR, Proteintech), rabbit anti-AKT (1:1000, 9272S, CST), rabbit anti-Phospho-Akt (Ser473) (1:1000, 9271S, CST), rabbit anti-Phospho-P38 MAPK (Thr180/ Tyr182) (1:1000, 8690 T, CST), Article Title: MRG15 decline in aged/injured MuSCs hinders regeneration via differentiation defects Article Snippet: Protein from cells or tissues was extracted by using RIPA buffer (P0013B, Beyotime) supplemented with Protease Inhibitor Cocktail (5892791001, Roche) and Phosphatase Inhibitor Cocktail (4906837001, Roche) immediately before use and the protein concentration was determined using a Pierce Coomassie Plus (Bradford) assay kit (23238, Thermo Fisher Scientific). .. The following antibodies and dilutions were used for Western blot analysis: rabbit anti-MRG15 (1:1000, 55257–1-AP, Proteintech), mouse anti-MyoG (1:1000, F5D, DSHB), mouse anti-MyoD1 (1:1000, M3512, Dako), mouse anti-MYH (sc-376157, Santa Cruz), mouse anti-TIP60 (1:1000, 10827–1-AP, Proteintech), mouse anti-P300 (1:1000, 83078–5-RR, Proteintech), rabbit anti-AKT (1:1000, 9272S, CST), rabbit anti-Phospho-Akt (Ser473) (1:1000, 9271S, CST), rabbit anti-Phospho-P38 MAPK (Thr180/Tyr182) (1:1000, 8690 T, CST), Article Title: Rab32/Rab38-positive Lysosome-Related Organelle degrades lipid droplet in hepatocytes by microautophagy Article Snippet: LAMP1 was detected using rat anti-LAMP1 (1D4B) (sc-19992, Santa Cruz Biotechnology; 1:100 for IF). .. S6 kinase was detected using rabbit anti-phospho-S6K (Thr389) (#9205S, Cell Signaling Technology; 1:1000 for WB) and other:Article Title: Hyperactivation of mTORC1 blocks stem cell fate transitions through TFE3-NuRD association Article Snippet: Article Title: Central Zika virus infection causes hypothalamic inflammation and persistent insulin resistance in adult mice Article Snippet: Primers used for ZIKV RNA amplification were: forward 5’-CCG CTG CCC AAC ACA AG-3’; reverse 5’-CCA CTA ACG TTC TTT TGC AGA CAT-3’; Reporter 5 ‘- / 56-FAM / AGC CTA CCT / ZEN / TGA CAA GCA ATC AGA CAC TCA A / 3IABkFQ / -3’ (Integrated DNA Technologies) as described by [ , ]. |